The core idea of metabonomics is to study the overall effect on the body produced by the exogenous material (drugs, environment, nutrition, etc.), which has the characteristics consistent with the holistic thinking of traditional Chinese medicine (TCM) treating disease.UPLC leads to better chromatographic peak resolution, considerable shorter analysis time and more sensitivity compared to HPLC, has been considered to have a more bright future in the research of metabonomics.Rhizoma Drynariae is one of the most frequently used TCM, which can replenish the kidney, strengthen the bones and promote the healing fracture.Up to now, there has been no report to demonstrate its mechanism of Kidney-Yang tonifying action.Neither has it been experimentally investigated whether nor how this herbal medicine affects the metabolism of whole body.