【摘 要】
：
Mango (Mangifera indica L.) is cultivated throughout the tropics as well as in subtropical areas and suffers from a number of diseases caused by bacteria, fungi and other agents.Because no efficient c
【机 构】
：
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haiko
【出 处】
：
中国植物病理学会2014年学术年会
论文部分内容阅读
Mango (Mangifera indica L.) is cultivated throughout the tropics as well as in subtropical areas and suffers from a number of diseases caused by bacteria, fungi and other agents.Because no efficient chemical control is available, mango bacterial black spot (MBBS;synonym mango bacterial canker) caused by Xanthomonas campestris pv.mangiferaeindicae (Xcmi;synonym X.citri pv.mangiferaeindicae) is a recurring limiting factor for mango industries in the Old World.The movement of infected, asymptomatic leaf, branch and fruit is a major means of pathogen dispersal.A reliable and sensitive diagnostic procedure is necessary for the safe movement of mango planting material.A set of primers based on the partial sequence of the hrpB gene was designed to develop a PCR assay for this pathogen.Primer pairs (XcmiHF and XcmiHR), which amplified a single specific band of 321 bp,were tested for specificity and sensitivity in detection DNA and cell from Xcmi.Amplication was positive only with genomic DNA from 12 Xcmi strains, no PCR products were observed with DNA from 10 other Xanthomonads and 7 other bacterial species.The sensitivity of detection was 2.4 pg of plasmid DNA, and 1.8 × 104CFU/mL cell.The primers also worked well for pathogen detection in direct PCR assays of Xcmi colonies grown on liquid medium and in PCR assays of total DNA from leaf, branch and fruit lesions.PCR assays proved to be relatively sensitive and could become very useful in detecting Xcmi in mango planting material.
其他文献
Rice blast disease, caused by the ascomycete fungal pathogen M.grisea (Hebert Barr, is a world-widely problem in rice growth and production.The rice blast system is a classical genefor-gene system, an
Rice plants have evolved a series of coordinated defense responses rendering them inaccessible to most would-be herbivores, but little is known about how it defends itself against herbivore attack.The
Many major blast resistance genes showed a high resistant level in rice-growing regions of Liaoning Province.However, the distribution of these genes in present rice leading cultivars of Liaoning Prov
Dickeya zeae (D.zeae) is the causal agent of devastating rice foot rot disease, which is an major concern of the agricultural industry in rice planting countries and regions.Cyclic di-GMP (c-diGMP) is
AvrRxo1, a type Ⅲ effector from Xanthomonas oryzae pv.oryzicola (Xoc), which causes bacterial leaf streak (BLS) in rice, can be recognised by non-host resistance protein Rxo1 and triggers a hypersensi
An antagonistic Bacillus amyloliquefaciens CGMCC6978, originally isolated from the permafrost soil samples collected from the Mohe, Heilongjiang province, China.It showed a strong biocontrol activity
Phytoplasmas are phloem-limited bacterial plant pathogens associated with approximately 1000 plant species worldwide, of which over 100 occur in China.In the study, four phytoplasma strains of chinabe
Xanthomonas oryzae pv.oryzae (Xoo) causes bacterial leaf blight (BLB), a devastating disease of rice in Asia countries.Xoo utilizes repertoires of transcriptional activator-like effectors (TALEs) to m
Plasmopara viticola, the causal agent of downy mildew, is considered one of the most important pathogens of grape.It has drastic effects on the host plants, on berries, but also on wine qualities and
Bacillus subtilis B96-Ⅱ is a broad-spectrum biological control strain isolated from compost extracts.It could effectively suppress the soil-borne fungal diseases of vegetables.Strain B96-ⅡG13 was obta