Background: Corynebacterium glutamicum is a non-pathogenic bacterium widely used in industrial amino acid production and metabolic engineering research.Although the genome sequences of some C.glutamicum strains are available,comprehensive comparative genome analyses of these species have not been done.Six wild type C glutamicum strains were sequenced using next-generation sequencing technology in our study.Together with 20 previously reported strains,we present a comprehensive comparative analysis of C.glutamicum genomes.