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Estrogen inhibits cyst breakdown and primordial follicle assembling of germ cells,but little is known about the underlying mechanisms.We aimed to analyze the effects of estrogen on the early development of mouse follicles using an in vitro ovary culture system and in vivo injection.Newborn mouse ovarian tissues were cultured in vitro for 2 or 4 days with estrogen of 0 M,10-8 M and 10-4 M,respectively,and neonatal mice were injected with 5 mg/kg/day estrogen.