【摘 要】
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OBJECTIVE To explore the optimal ultrasonic-assisted process and increase the yield of alkaloids from Piper longum L..METHODS Optimal ultrasound-assisted extraction (UAE) process of alkaloids from P l
【机 构】
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Beijing Key Lab of TCM Collateral Disease Theory Research and School of Traditional Chinese Medicine
【出 处】
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中国药学会第十三届青年药学科研成果交流会
论文部分内容阅读
OBJECTIVE To explore the optimal ultrasonic-assisted process and increase the yield of alkaloids from Piper longum L..METHODS Optimal ultrasound-assisted extraction (UAE) process of alkaloids from P longum was investigated by orthogonal test and the concentration of ethanol, ratio of material to liquid, duration of extraction, ultrasonic frequency and temperature were optimized based on single factor tests.The content of total alkaloids was determined by UV-Vis spectrophotometer and the contents of piperine and piperlongumimne were determined by high performance liquid chromatography (HPLC).RESULTS The optimal ultrasound-assisted extraction conditions are as follows: the concentration of ethanol is 75%, the ratio of material to liquid is 1:10, the ultrasonic extraction time is 50min, ultrasound temperature is 40℃ and frequency is 45 kHz.The yields of total alkaloids, piperine and piperlonguminine from P longum obtained by UAE were higher than that extracted by reflux extraction.CONCLUSION Ultrasound-assisted extraction method can shorten the time of extraction and improve the yield of alkaloids from P.longum.
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