Two new, at primary sequence and protein structure levels similar, laccase encoding genes from the white rot basidiomycete Trametes gibbosa (Tg) are cloned and sequenced.Both genes Tg-lacl and Tg-lac2contain 10 introns and a full open reading frame (ORF) of 1563 nucleotides which code for a 520 amino-acid precursor polypeptide.In both genes, the position of both ten introns is highly conserved, intron splicing follows the general eukaryotic rule-gta/g...t/cag except the sixth intron of Tg-lac2 which begins with-gcg.Only five and six putative internal lariat formation sites in Tg-lacl and Tg-lac2 match the consensus sequence CTRAY, respectively.The full-length cDNAs of Tg-lac1 and Tg-lac2 consist of 1744, 1873 nucleotides excluding the poly(A) tail which contain a 59 and 73 nucleotides of 5 untranslating region, and a 122 and 237 nucleotides of 3 untranslating region, respectively.