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Bacteria play pivotal roles in degradation of pig manure(PM).Microbial fermentation bed(MFB)was introduced to recycling utilization of agricultural byproducts and pig manure(PM).By 16S high throughput sequencing technology,microbial population dynamics of PM under in situ fermentation on the MFB system in situ fermentation PM at different stages were evaluated.Physicochemical factors,including organic carbon,nitrogen,phosphorus,potassium and 3-methylindole(3-MI)contents were also determined.Bacterial community structure and composition significantly varied at different stage of PM.Fresh PM had most abundance bacterial taxa among five samples.Pig manure with short-term fermentation(0,5 and 10 d)had similar bacterial community,separately from PM with long-term fermentation(15 and 20 d).In long-term fermentation litters,organic degradation strains as Corynebacterium,Bacillus,Virgibacillus,Pseudomonas,Actinobacteria,Lactobacillus,Pediococcuswere the dominate genus.Meanwhile,3-MI concentration of PM was reduced by 84%after 20 d in situ fermentation.The 3-MI concentration in the PM could be gradually decreases in the MFB.These results provide appropriate information for biodegradation of PM.