【摘 要】
：
The recent description of the complete genomes of the two most pathogenic species of Brucella opens the way for genome-based analysis of the antigenicity of their proteins.In the present study, we des
【机 构】
：
Department of Bacterial Diseases, Division of Communicable Diseases and Immunology, Walter Read Army
【出 处】
：
2005 WHTS3rd Annual Congress of International Drug Discovery
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The recent description of the complete genomes of the two most pathogenic species of Brucella opens the way for genome-based analysis of the antigenicity of their proteins.In the present study, we describe a bench-level High-Efficiency Cloning System that allows cloning and expression of large numbers of Brucella proteins based on genomic sequence information.Purified proteins are produced with high efficiency in a protein-array format conducive to analysis of their sero-reactivity against serum from immunized animals.This method is applicable at small and large scale.While it does not require robotics, the format is amenable to robotic implementation for all aspects of the process and subsequent analysis of protein characteristics.This method will allow development of new reagents for diagnosis and vaccine development for Brucella, an important zoonotic disease and biothreat agent.
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