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The aim of this study was to examine the role of microbubble (MB) diameter on cavitation-mediated anti vascular and anti-tumor effects in a mouse model of human pancreatic cancer.A polydisperse MB contrast agent formulation (TS-P) with a mean number diameter of 1.9 um was depleted in small diameter particles by differential centrifugation, producing an "up-sized" size distribution (TS-PL) possessing a mean diameter of 2.9 um.