Morphology and Pathogenicity of Colletotrichum capsici Causing Grape Anthracnose in Guangxi, China

来源 :中国植物病理学会2014年学术年会 | 被引量 : 0次 | 上传用户:tt7506
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  Anthracnose is one of the most common fungal diseases of grape cultivars (Vitis vinifera)in most parts of China.Although worldwide anthracnose is reported to be caused by Elsinoe ampelina (de Bary) Shear (anamorph Sphaceloma ampelinum de Bary), in our previous research another pathogen is also the causal pathogen.The objective of this study was to examine the pathogens morphological observation, pathogenicity and analysis of nucleotide sequence of internal transcribed spacer (ITS).The pathogens of grape anthracnose were isolated and purified from susceptible disease leaves of grape cultivar cultivated in Nanning, Guangxi, China by conventional tissue method.3 types of pathogens were isolated from the diseased grape leaves.Analysis of ITS showed that pathogen NN002 was identified as the pathogen of Colletotrichum capsici.Its conidium is falcate, single, colorless with size 20 ~30μm × 3 ~6μm.It was pale villous first, and then turned into dark gray even blackish green later.It showed round tidy edge and dark gray aerial hyphae.Its colony wheet was relatively clear.The matrix turned into a dark brown later and many small particles scattered on it.Spraying inoculation of spore suspension of the pathogen infect "Thompson seedless" grapes young leaves, young tips and young fruits, all of them showed a typical grape anthracnose disease symptoms after 7d.The conclusion is that Colletotrichum capsici is also a pathogen of grape anthracnose in Nanning, Guangxi.
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