【摘 要】
:
A rapid method for determination of poison in human blood by supercritical fluid extraction(SFE)-Supercritical Fluid Chromatograph(SFC)-tandem mass spectrometry was build.The simple pretreatment metho
【机 构】
:
Shimadzu(China)Co.,LTD.Shanghai 200052,China
【出 处】
:
43rd International Symposium on High Performance Liquid Phas
论文部分内容阅读
A rapid method for determination of poison in human blood by supercritical fluid extraction(SFE)-Supercritical Fluid Chromatograph(SFC)-tandem mass spectrometry was build.The simple pretreatment method as following: the blood sample was dropped 20 μL to a Dry Blood Spot(DBS)card,then holed 3 mm internal diameter dry blood spot,and put it into the SFE vessel to analysis by Shimadzu online SFE-SFC-MS system.Some of SFE conditions,like SFE pressure,modifier solution,and SFE temperature were investigated.By the suitable SFE condition,the linearity,limit of detection,recovery and repeatability were also studied.The results showed that the linear ranges for six benzodiazepine medicines were 1~1000 ng/mL,the percent recovery of medicines were between 80~110%and the limit of detections for most medicines in specimens were between 1~10 ng/mL.The entire method is simplified,fast,accurate,highly sensitive,and is a new method for determination of components and selected metabolites of benzodiazepine medicines in human blood.
其他文献
Hydrophilic interaction chromatography(HILIC) is a very popular method providing the retention and separation of polar compounds,which are difficult to analyze by reversed-phase HPLC.Since the separat
In recent years it has becoming increasingly popular to use pH gradients as a platform method for the separation of proteins when using ion exchange columns for quality control characterization of pro
The main composition of edible oil(vegetable oil or animal fat) is triacylglycerol(TAG).There is difference of kind and content of TAGs in different edible oils as TAG esterified with different fatty
The Chlor-alkali industry is one of the largest electrochemical operations in the world,the main products being chlorine and sodium hydroxide produced simultaneously by the electrolysis of sodium chlo
Bromine and bromine derivatives are produced from sea brine.Bromine is used in flame retardants,drilling fluids,gasoline additives,pesticides etc.The most important group of flame retardants is bromin
Citalopram,chemically 1-(3-dimethylaminopropyl)-1-(4-fluorophenyl)-1,3-dihydroiso-benzofuran-5-carbonitrile,is a serotonin reuptake inhibitor(SSRI).The pharmacological activity lies predominantly in i
Recently,a number of researches have been devoted to the analysis and detection of trace analytes in complex matrix.In the research,polypyrrole/graphene(PPy/G) composite coating was prepared by a faci
In this work,a microfluidic device coupled to liquid chromatography-mass spectrometry(LC/MS) for implementing drug assay and metabolic analysis of glioma stem cells was developed.An integrated two-lay
An automatic HPV typing method on microchip electrophoresis(MCE) system has been developed that takes the advantage of high-throughput and rapid separation of DNAs.In the method,different kinds of HPV
Zinc pyrithione(ZPT) and climbazole(CBZ) as anti-fungal agents are widely used in marketed shampoos for antidandruff(AD) treatment.Its claimed that they offer the benefits of inhibiting microbe growth