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BAC(biological actived carbon,BAC)in drinking water treatment process can affect the structure of the microbacterial.Culturable bacteria in one of the water supply system in Shanghai were isolated and identified by 16SrRNA gene sequencing.The multidrug resistance in drinking water for culturable bacteria and their change and mechanism in water supply system were analyzed by using five kinds of antibiotic.The results show that Bacillus sp.,Arcicella rosea and Sphingomonas sp.are existed in all samples of the process.The multidrug resistances of these three bacteria are enhanced after carbon filtration and water distribution.Bacillus spshowsthe strongest antibiotic resistance.Real-time PCR was used to quantitatively evaluate the concentration of three integrons and 9 transposons.The results show that the NO.Of detected genes increased from 100 to 124 after BAC filter,both the absolute and the relative concentrations of Sulfonamide,Aminoglycoside,other/efflux and mobile genetic elements increase obviously,which means that BAC filter and water distribution system play an important rolein influencingantibiotic resistance in the water treatment process.