【摘 要】
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Characteristics of enzyme transition state analogues are reviewed for a range ofreactions.Tetrafluoroaluminate is widely employed as a transition state(TS) mimic but itnecessarily imposes octahedral g
【机 构】
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Krebs Institute,Department of Molecular Biology and Biotechnology,The University of Sheffield,Sheffi
【出 处】
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第九届IUPAC化学生物学国际研讨会暨第八届世界华人药物化学研讨会
论文部分内容阅读
Characteristics of enzyme transition state analogues are reviewed for a range ofreactions.Tetrafluoroaluminate is widely employed as a transition state(TS) mimic but itnecessarily imposes octahedral geometry on the assembled complexes whereas phosphoryltransfer involves trigonal bipyramidal,tbp,geometry.Trifluoromagnesate,MgF3-,providesthe near-ideal solution,delivering tbp geometry and correct anionic charge),2 TS analoguesfor a range of kinases,mutases,and phosphatases provide a detailed description of mechanismfor phosphoryl group transfer,supporting the concepts of the anionic shield for ground statestability and of charge balance for their TSs and concerted-associative pathways forbiocatalysis.
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