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A sensitive signal-off photoelectrochemical(PEC)sensing platform for myoglobin(Myo)detection based on Mn-doped CdS quantum dots-sensitized two-dimensional(2D)layered g-C3N4-MoS2 heterojunction coupled with CuO labeled anti-Myo(anti-Myo-CuO)conjugates as signal amplification tags was developed.