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目的了解澳门地区献血者标本中,HIV、HBV和HCV血清学检测为阴性,核酸检测为阳性的发生频率。方法采用回顾性调查方法,对2007年5月—2010年6月已经过病毒血清学检测(包括HBsAg、抗-HCV及抗-HIVⅠ/Ⅱ)、排除了病毒血清学筛查阳性后余下的40149个阴性献血者标本,做HIV、HBV及HCV的核酸筛检。结果共筛检测29个标本呈阳性反应,将它们做病毒区分检测:没有发现HIV及HCV阳性个案,确认为HBV阳性24例,其余5例为未能区分病毒种类。进一步追踪调查:在初筛确定为HBV核酸阳性的24例中,13例追踪标本HBV核酸检测呈阴性,2例(2例2次,1例3次)持续呈阳性。结论 1)澳门地区属乙型肝炎的高发区,实施病毒NAT筛检提高了血液的安全水平;2)追踪隐性乙型肝炎患者的HBV DNA变化和血清学检测,除了是关注献血者的健康状况外,亦为制定献血者筛除政策提供依据。
Objective To understand the frequency of serological tests of HIV, HBV and HCV in blood donors in Macao. Methods The retrospective investigation method was used to detect serological tests (including HBsAg, anti-HCV and anti-HIV / Ⅱ) from May 2007 to June 2010 and the remaining 40149 A negative donor samples, do HIV, HBV and HCV nucleic acid screening. Results A total of 29 specimens were screened for positive results. They were tested for virus differentiation. HIV and HCV positive cases were not found, 24 cases were positive for HBV. The remaining 5 cases were unable to distinguish the virus types. Further follow-up survey: Of the 24 cases positive for HBV nucleic acid screening, 13 cases were negative for HBV DNA test, and 2 cases (2 cases in 2 cases and 1 case in 3 cases) continued to be positive. Conclusions 1) In the high incidence area of hepatitis B in Macao, the implementation of virus NAT screening improves the blood safety level; 2) To track HBV DNA changes and serological tests in patients with occult hepatitis B, except that it is concerned with the health of blood donors In addition, it also provides a basis for formulating a blood donation screening policy.