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采用放射性核素８６Ｒｂ测定小鼠心肌营养性血流量的方法。用地奥心血康８０ｍｇ／ｋｇ，ｉｇ或４０ｍｇ／ｋｇ，ｉｐ，均能增加心肌营养性血流量。＊Ｐ＜０．０１。讨论小鼠心肌对８６Ｒｂ摄取量的大小，在很大程度上取决于心肌内开放毛细血管的数量和面积，在一定条件下，心肌血流量愈大，对８６Ｒｂ的摄取量愈多，所以，心肌对８６Ｒｂ的摄取量可以代表心肌营养血流量。采用８６Ｒｂ测定心肌营养性血流量报道较多，但处理心脏的方法有组织研磨法［２］，组织消化法［３］和直接测定法［１，４］。本文采用直接测定法［１］，操作比较简便。关于注射８６ＲｂＣｌ后处死动物时间的选择，施新献认为心脏对８６Ｒｂ的摄取是一个动态过程，在注射后３０ｓ至１５ｍｉｎ的阶段内，心肌８６Ｒｂ含量高且平稳。因此，选择静注后３０ｓ作为观察心肌对８６Ｒｂ摄取能力的时间标准是比较合适的［５］。地奥心血康４０，８０ｍｇ／ｋｇ，ｉｐ和８０ｍｇ／ｋｇ，ｉｇ，均有不同程度地增加小鼠心肌营养性血流量作用，其中８０ｍｇ／ｋｇ，ｉｐ与双嘧达莫１００ｍｇ／ｋｇ，ｉｐ能显著增加小鼠心肌营养性血流量，其作用强度相似，与生理盐水组比较Ｐ＜０．０１。但地奥心血康２００ｍｇ／ｋｇ１次ｉｇ，反而减少心肌?
Method of using radioactive nuclide 86Rb to measure cardiac nutritional blood flow in mice. Austria Austrian blood Xue Kang 80mg / kg, ig or 40mg / kg, ip, can increase myocardial nutritional blood flow. * P <0.01. Discussion Mouse myocardium uptake of 86Rb size, largely depends on the number and area of open intramyocardial capillary, under certain conditions, the greater the myocardial blood flow, the more the 86Rb uptake, so the myocardial The intake of 86Rb can represent myocardial nutrient blood flow. 86Rb determination of myocardial nutritional blood flow is reported more, but the heart of the method of tissue grinding method [2], tissue digestion [3] and direct determination [1,4]. In this paper, direct determination [1], the operation is relatively simple. Regarding the choice of time to sacrifice animals after injection of 86RbCl, Shi Xinxian suggested that the heart’s uptake of 86Rb was a dynamic process with high and steady 86Rb levels in the myocardium from 30s to 15min after injection. Therefore, 30s after intravenous injection as a time standard for observing myocardial uptake of 86Rb is more appropriate [5]. Diaoqangkang 40, 80mg / kg, ip and 80mg / kg, ig, respectively, increased myocardial nutritional blood flow to varying degrees, of which 80mg / kg, ip and dipyridamole 100mg / kg, ip can significantly increase the small Nutritional blood flow in rat myocardium was similar to that of saline group (P <0.01). However, Austrian blood Cang Kang 200mg / kg1 times ig, but to reduce myocardial?