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Soft-shelled turtle iridovirus (STIV) is the causative agent of serious diseases in the farmed soft-shelled turtle (Trionyx sinensis).Here we determined the complete nucleotide sequence of STIV genome.The STIV genome is 105890 bp in length with a base composition of 55.1% G+C.Computer assisted analysis revealed that STIV contained 105 potential open reading frames (ORFs) which encode polypeptides ranging from 40 to 1,294 amino acids and 20 MicroRNA candidates.