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Background:Porcine circovirus type 2 (PCV2),the causative agent of postweaning multisystemic wasting syndrome (PMWS),is a serious economic problem for the swine industry in China.In this study,we investigated the genetic variation of PCV2 in China using strains isolated from 2004-2008.Viruses were isolated from samples collected from pigs with multi systemic lesions and clinical signs of PMWS from different regions of China,and the genomes of these viruses were sequenced.The assembled sequences were used to define the genotypes of these strains;PCR-RFLP methodology was used to distinguish isolates and capture ELISA was used to demonstrate the antigenic changes resulted from ORF2 gene mutation of the isolates.