【摘 要】
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N-Nitrosonornicotine(NNN)is considered to be one of the most carcinogenic compounds of the four conventionally measured tobacco-specific N-nitrosamines(TSNAs).In order to evaluate the significance of
【机 构】
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Shanghai Tobacco Group Corporation of CNTC,99 Wansheng South Street,Tongzhou District,Beijing 101121
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N-Nitrosonornicotine(NNN)is considered to be one of the most carcinogenic compounds of the four conventionally measured tobacco-specific N-nitrosamines(TSNAs).In order to evaluate the significance of metabolic activation for the carcinogenic potential of NNN,its catalysis by different phase I enzymes and its interaction with nicotine and nicotine-derived TSNAs need to be investigated.Using an in vitro model system,NNN was found to interact with various cytochrome P450 enzymes,predominantly CYP2A13.Mass-spectrometric analysis confirmed the presence of various predicted NNN metabolites,including 4-hydroxy-1-(3-pyridyl)-1-butanone(HPB)and 4-hydroxy-4-(3-pyridyl)-butyric acid(hydroxy acid)but little amount of 4-oxo-4-(3-pyridyl)butanal(OPB),which was somewhat different from in vitro NNK metabolism.Addition of nicotine,N-Nitrosoanatabine(NAT),N-Nitrosoanabasine(NAB)resulted in a competitive inhibition for NNN metabolism.The inhibition constant Ki value was calculated as 0.98 μM(nicotine),1.37 μM(NAT),0.71 μM(NAB)for HPB formation,1.35 μM(nicotine),1.35 μM(NAT),1.01 μM(NAB)for hydroxy acid formation and 8.40 μM(nicotine),3.40 μM(NAT),3.04 μM(NAB)for OPB formation,respectively.These results implied that CYP2A13 is the most efficient enzyme to metabolize NNN in vitro and structurally similar tobacco constitutes including nicotine,NAT and NAB influence the metabolic activation of NNN,which may further interfere in its carcinogenicity.
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