Cell-Free Circulating DNA as a novel diagnostic marker for gastric cancer

来源 :第十三届全国肿瘤生物治疗学术会议 | 被引量 : 0次 | 上传用户:diliwer3
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
  Background: In our previous study, a novel and sensitive method for quantifying circulating cell-free DNA (CFD) in human blood was established and tested for its ability to predict gastric cancer (GC) in patients.In the present work, we first used the branched DNA (bDNA)-based Alu assay to measure CFD levels, and then investigate CFD expression in the sera of GC patients in an attempt to explore the clinical significance of CFD in improving the early differential diagnosis of GC and monitoring GC progression.Methods: Serum samples were collected randomly from 124 unselected GC patients, 32 patients with gastric benign disease (BGD), and 92 healthy controls.Alu-based CFD concentrations were detected by branch DNA (bDNA).Concentrations of carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9), carbohydrate antigen 72-4(CA72-4), carbohydrate antigen 50(CA50) were detected by ARCHITECT assay.Results: There was a significant difference in CFD concentrations between GC patients, BGD patients and healthy controls (P<0.05).A significant correlation was discovered between CFD and CEA (r=-0.197, P<0.05) or CA50 (r=0.206, P<0.05), but there was no significant correlation between CFD and CA199 (r=-0.058, P >0.05) or CA724 (r=0.016, P>0.05).In addition, CFD concentrations in stage I GC patients were significantly higher than BGD patients and healthy controls (P<0.05), but for conventional biomarkers except CA72-4, there were no significant difference in CEA, CA19-9 and CA50 between the three groups (P>0.05).Our analysis showed that CFD was more sensitive than CEA, CA19-9, CA72-4 or CA50 in early differential diagnosis of GC.Conelusions: Compared with CEA, CA19-9, CA72-4 and CA50, CFD may prove to be a better biomarker for the early differential diagnosis of GC, thus providing a potentially specific tool for aided diagnosis of GC, and a sensitive biomarker for monitoring progression and predicting prognosis of GC.
其他文献
家用录像机已由单电机皮带传动发展到了由独立的伺服电机直接驱动磁鼓和主导轴,不仅使录放图像稳定,而且也便于伺服到统一的标准,提高了录像机的通用性。随着录像机的新录放
在高职院校中,双创教育的落实需要以专业教育为载体,它是专业教育的继续延伸.在社会人才需求的变化下,专业教育、双创教育的融合是一类必然趋势,要保障教育融合的实际效果,便
  Hypothalamus arcuate nucleus(ARC)is a main site for neurons expressing neuropeptides involved in feeding and energy expenditure behavior.The molecular mecha
近年来,我国社会综合国力得到了显著的提升,从而为各个领域的发展带来了良好的机遇,这样就使得各个行业对专业人才的需求更加的迫切.中职院校开展思想政治教育工作不但与民众
英国《金融时报》与晋华永道会计师事务所日前联合公布了2002年全球最受尊敬企业名单,TCL入选“中国最受尊敬企业”。世界“最受尊敬企业”排行榜是公司声誉的著名报告单,由
中职学校相对普通高中而言,课业负担较轻.基于中职学生的具体情况,学生除了要求掌握必须的操作技能之外,更要加强学生的德育教育.虽然学校有专门的德育课,但数量有限,远远达
一、引言Viterbi 算法是一种有效的纠错译码算法,它具有显著的检错和纠错能力。但由于算法复杂、运算量大,长期以来很难应用于高速实时处理系统。近年来,随着微处理机技术的
  心脏的发育过程伴随着一系列精确的细胞增殖、分化及凋亡过程,其相关基因的调控异常将导致胚胎心脏发育成畸形心脏.CXXC 锌指蛋白5(CXXC5)是CXXC 家族成员,具有物种进化
荔枝采后果皮自然地变褐变干,虽未影响其丰味,但都严重地降低其商品价值。采用二氧化硫和盐酸处理可使果皮获得均匀的红色。方法是:采果当天 The litchi plum naturally br
在中职阶段,信息技术所具有的学科地位比较显著,对于培养信息化人才具有一定的促进作用.在开展教学活动的过程中,教师需要积极地革新育人思想,将以学为主的战略思想有效地贯