【摘 要】
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Pregnancy-associated plasma protein A (PAPP-A) is an important serum marker for first trimester screening.Its weekly median value varies with ethnicity.A one-step time-resolved fluoroimmuno assay (TRF
【机 构】
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Prenatal Diagnosis Centre, Guangdong Women and Children Hospital, Guangzhou, Guangdong, China
【出 处】
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广东省遗传学会第九届代表大会暨学术研讨会
论文部分内容阅读
Pregnancy-associated plasma protein A (PAPP-A) is an important serum marker for first trimester screening.Its weekly median value varies with ethnicity.A one-step time-resolved fluoroimmuno assay (TRFIA) using two monoclonal antibodies against PAPP-A with Eu(3+) chelates as labels has been developed.Using the assay described here, we evaluated 5,301 normal serum samples from Chinese women at 7-13 weeks of gestation.The detection limit using this assay was 1.2 mIU/L, and the maximum detection range was up to 10,000 mIU/L.The intra-assay and inter-assay coefficients of variation were <3.0% and <5.0%, respectively, and the mean recovery rate was 98.0%.PAPP-A concentrations measured in 516 maternal serum samples correlated well with those obtained by Dissociation-Enhanced Lanthanide Fluorescent Immunoassay (DELFIA) PAPP-A assay (r=0.988, P<0.001).The medians for 7-13 weeks of maternal serum PAPP-A were higher in the women from China compared to reports from other countries.The present assay possesses accuracy and high sensitivity and exhibits great potential for the clinical analysis of PAPP-A.Our investigation on themedian concentrations of PAPP-A will help establish reference values that are specific for China and study the importance of ethnic factors in biochemical screening.
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