【摘 要】
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We thought to determine the effect of ADAMTS-7 (a disintegrin and metalloproteinase with thrombospondin motifs) in angiogenesis.Overexpression of ADAMTS-7 in human umbilical vein endothelial cells (HU
【机 构】
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Department of Physiology and Pathophysiology,School of Basic Medical Sciences,Peking University,Beij
【出 处】
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International Conference for Physiological Sciences 2012(201
论文部分内容阅读
We thought to determine the effect of ADAMTS-7 (a disintegrin and metalloproteinase with thrombospondin motifs) in angiogenesis.Overexpression of ADAMTS-7 in human umbilical vein endothelial cells (HUVECs) significantly inhibited endothelial cell proliferation (ADAMTS-7 vs.control: OD.0.1778±0.0034 vs.0.3597±0.0343, P<0.001), migration (distance: 96.0±0.9 vs.131.3±7.7 μin, P<0.01) and tube formation (average length: 35880±1892 vs.58540±1660 plex, P<0.0001).Reciprocally, ADAMTS-7 inhibition by neutralization antibody significantly abolished the anti-angiogenic effect of ADAMTS-7 (100.0%±5.3% vs.132.0%±1.2%,P<0.05).In addition, perivascular administration of ADAMTS-7 adenovirus to rat carotid arteries greatly reduced the average length of outgrown microvessels in the aortic ring assay (ADAMTS-7 vs.control: 370.7±41.4 vs.629.3±43.1 pm, n=3, P<0.05).Injection of matrigel plugs with HUVECs overexpressing ADAMTS-7 into C57/BL6 mice suppressed neovascularization by 25% in comparison with that induced in control group (number of cells per field: 150.2±39.1 vs.205.9±50.9, n=4, P=0.052).Taken together, these data suggest ADAMTS-7 as a novel endogenous angiogenesis inhibitor.
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