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Multiplex detection is in urge need due to its important role in disease diagnosis,gene expression and drug screening.In this work,a highly selective and sensitive surface enhancement Raman scattering(SERS)-based binary encoding strategy for multiple detection of antigens has been developed.The as-designed binary encoding was composed of photonic crystal bead(PCB)and SERS nanotag.The SERS nanotag was fabricated by directly absorbing Raman active molecules(reporter molecules,RMs)onto Au nanoparticles(GNPs)via electrostatic interaction and encapsulating them in a polyethylene glycol(PEG).Owing to the fingerprint properties of Raman molecule with narrow half peak width,the resulting encoding number was greatly increased.More importantly,results of sensitivity analysis indicated that the detection system exhibited a good analytical performance with a wide detection linear range from 100 μg/mL to 10 pg/mL,and a low detection limit of 9.4 pg/mL.This SERS-based binary encoding would provide great potential for effective and sensitive multiple antigens detection.