The growing demands for high-throughput analysis in post-genomic research, such as in proteomics or metabolomics, has stimulated the development of high-speed separation techniques and tools[1,2].Monolithic column features several significant advantages compared with conventional microparticles-packed column: low-pressure drop through the column, fast mass transfer kinetics, and high loading capacity.Recently, it has been an attracting topic for development of diverse monoliths for biomolecules.