【摘 要】
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IFN-α is a cytokine that plays crucial role in innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control.IFN-α is also a key activator of macrophages.In p
【机 构】
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Central Laboratory, China Medical University, No. 92, North Second Road, Heping District,Shenyang 11
【出 处】
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第三届东北三省暨内蒙古自治区免疫学学术会议
论文部分内容阅读
IFN-α is a cytokine that plays crucial role in innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control.IFN-α is also a key activator of macrophages.In present study, we studied detailed modulation of IFN-α on phenotypic and functional maturation of murine bone marrow derived dendritic cells (BMDCs).Phenotypic and functional maturation of BMDCs was evaluated by light microscope, flow cytometry(FCM), transmission electron microscopy(TEM), cytochemistry method, acid phosphatase activity(ACP), FITC-dextran bio-assay and enzyme linked immunosorbent assay (ELISA).We elucidated that IFN-α up-regulated the expression of MHCⅡ, CD40, CD80, CD83 and CD86 molecules on BMDCs, down-regulated activity of pinocytosis and phagocytosis by BMDCs, induced higher levels of IL-12 and TNF-α secreted by BMDCs.It is therefore confirmed that IFN-α can effectively promote the maturation of BMDCs.Our study provides more evidence and rationale on future application of IFN-α for enhancing host immunity and potent adjuvant in the design of DC-based vaccines.
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