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Context: Silkworm excrement,as traditional Chinese medicine,has the capabilities of antioxidant,hypoglycemic,anti-tumor and other biological activities.Objectives: The research emphasizes on antioxidant activity of different solvent extracts of silkworm excrement,and the flavonoids content in the extract were also determined.Lay a foundation for the comprehensive utilized of Silkworm excrement.Materials and methods: Five different polarity solvent were used to extract 5 g silkworm excrement respectively,and the flavonoids content and free radical scavenging ability of extracts were determined.Results: The strength of antioxidant activity of Silkworm excrement extracts from different solvents can be listed in an order of aqueous extract (AE) > ethanol extract (EE)> chloroform extract (CE) > ethyl acetate extract (EAE) > petroleum ether extract (PEE).The flavonoids content in these different extracts are as the following: aqueous extracts (29.498mg/g),ethanol extract (15.025 mg/g),ethyl acetate extract (10.181 mg/g),chloroform extract (8.506 mg/g),petroleum ether extract (5.875 mg/g).