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The macaque is a protected second class animal in China.To evaluate the distribution and public heal th significance of entamoeba species in captive macaques in China,148 macaques fecal samples were collected from macaques colony in Guangxi,all samples were examined used by polymerase chain reaction (PCR).The o verall prevalence of Entamoeba spp.was 65% (96/148),E.dispar were detected in 59 of 96 microscopic:positive samples (61.46%).E.coli were detected in 65 of 96 microscopic:positive samples (67.71%).Among them,the n umber of mixed infection was 32.No DNA of E.histolytica and other entamoeba species was found.These result s demonstrated that the pathogenic E.histolytica was absent in this colony,regardless of the high degree ofprev alence of other amebas.This study complements the limited information available on the animal hosts of Entam oeba spp.in China.