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Objective: We investigated the protective effects of the herbal formulation in androgen-deprived rat bladder.Methods: Male rats aged 8 weeks were randomly divided(n=8 in each): sham operation only(normal control),androgen-deprived only(androgen-deprived control),androgen-deprived followed by treatment with 200 and 400 mg/kg.After leuprorelin 0.5 mg/kg was subcutaneously injected in androgen-deprived groups,oral administration of either distilled water(normal control,androgen-deprived control)or herbal formulation(treatment groups)was continued for 4 weeks.Serum testosterone level,RhoGEFs,nitric oxide(NO)-cyclic guanosine monophosphate(cGMP)related parameters,oxidative stress,and histologic change were evaluated after treatment.Results: Treatment with the herbal formulation(Ⅰ)increased the serum testosterone levels;(Ⅱ)restored the expression of RhoGEFs,eNOS and nNOS;(Ⅲ)increased the expression of superoxide dismutase;and(Ⅳ)decreased bladder fibrosis.Conclusions: Our results suggest that a positive influence on molecular change in the urinary bladder would be attributable to an antioxidant effect or to an elevation in NO-cGMP activity.