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Background to evaluate phylogenetic analysis based on IGS1 and ITS2 sequencing was used for molecular identification of Trichosporon spp., while compared with the standard BLAST analysis.Methods 54 Trichosporon type and reference strains consisting of 39 species and 232 clinical strains which were formally identified as Trichosporon spp.were sequenced for IGS1 and ITS2 regions.All the