Ca2+ dependent nanomolar affinity to 3-phosphoadenosine-5-phosphate enables yeast 3, 5-bisphosphate

来源 :第十一届全国酶学学术讨论会暨邹承鲁诞辰90周年纪念会 | 被引量 : 0次 | 上传用户：lovemy521

【摘要】

：

　　Affinity chromatography is one of the most popular methods for protein purification.Yeast 3,5-bisphosphate nucleotidase (YND) had been found hydrolyzing 3-p

【作者】

：

Yang Yang Jianhui Ma Yilin Yang Ting Wang Xiao Zhang Yanxing Wang Ling Yang Thomas Leyh Meihao Sun

【机构】

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CollegeofChemistryandLifeSciences,ZhejiangNormalUniversity,Jinhua,321004DepartmentofM&I,AlbertEinste

【出处】

：

第十一届全国酶学学术讨论会暨邹承鲁诞辰90周年纪念会

【发表日期】

：

2013年5期

【关键词】

：

affinity chromatography nucleotidase adenosine 5'-phosphosulfate kinase pro

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　　Affinity chromatography is one of the most popular methods for protein purification.Yeast 3,5-bisphosphate nucleotidase (YND) had been found hydrolyzing 3-phosphoadenosine 5-phosphate (PAP) with submicromolar Km, which indicated the tight interactions between them.In order to explore the feasibility of YND as a protein purification affinity tag, YND was characterized with PAP as substrate.

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Ca2+ dependent nanomolar affinity to 3-phosphoadenosine-5-phosphate enables yeast 3, 5-bisphosphate

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