【摘 要】
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This study presents the effects of alternating current electric field (10KHz,2000V/cm) on the fluorescence labeling of microalgae by two lipid probes: Nile red and LipidTOX.Microalgae produce profuse
【机 构】
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Department of Chemical Engineering,National Cheng Kung University,Tainan,Taiwan
【出 处】
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第八届全国微全分析系统学术会议、第三届全国微纳尺度生物分离分析学术会议暨第五届国际微化学与微系统学术会议
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This study presents the effects of alternating current electric field (10KHz,2000V/cm) on the fluorescence labeling of microalgae by two lipid probes: Nile red and LipidTOX.Microalgae produce profuse neutral lipids that can be transformed to biodiesel by esterification.Fluorescence detection of microalgae neutral lipids is established in 1970s; however,it has yet to become the universal method for lipid quantification.Based on our previous experimental results,electric field can increase fluorescence intensity of microalgal lipids but the phenomenon is somehow ambiguous because of photo-bleaching in the static analysis (Fig.1).Therefore,this study aims to use flow-through analysis (Fig.2) to reduce photo-bleaching to reveal the abilities of electroporation in facilitating the transport of fluorogenic dyes into Chlorella vulgaris cells.The electroporation is presumably to assure fast and uniform fluorescence labeling of cellular lipids.Figure 3 and 4 show that AC electric field at 10 KHz and 2000 V/cm can increase the fluorescence intensity by 64 % with Nile red and 13% with Lipid TOX compared with the control (0 V/cm).The results show the great potential of flow-through electroporation in improving the quality of fluorescence detection of cellular lipids.This work is funded by the National Science Council of Taiwan via NSC 101-2311-B-006 -003 and by the Ministry of Education,Taiwan,R.O.C.via The Aim for the Top University Project to the National Cheng Kung University (NCKU).
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