Due to the relatively long persistence of DNA after cell death in the range between days to 3 weeks,DNA-based diagnostics tend to overestimate the number of live cells.EMA is a kind of nucleinic acid stain.EMA is selectively and covalently label dead cells in the presence of live cells.DNA covalently bound to EMA can not be LAMP amplified.Thus,only DNA from viable cells can be detected.To solve the DNA-based diagnostics problem,a new method of ethidium monoazide (EMA) in combination with loop-mediated isothermal amplication (LAMP) for detection the viable/dead the major food-borne salmonella was developed.