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目的:探讨低功率光动力治疗能更有效诱导宫颈癌细胞(Hela)凋亡的发生机制。方法:将光敏剂Photofrin 5μg/ml与Hela细胞共孵育16h后,应用波长为633nm氩离子激光器进行照射,能量密度3J/cm2,功率密度分别为3和30mW/cm2,采用荧光探针标记技术和细胞器的荧光强度比值法对细胞内
Objective: To investigate the mechanism of low-power photodynamic therapy more effectively inducing apoptosis of cervical cancer cells (Hela). Methods: Photofrin 5μg / ml was incubated with Hela cells for 16h, then irradiated by 633nm argon ion laser with the energy density of 3J / cm2 and the power densities of 3 and 30mW / cm2, respectively. The ratio of fluorescence intensity of organelles to intracellular