Initialization of immobilizer is a key process before vehicles are going off line, related to the success of matching immo- bilizer and igniting the engine. By analyzing mainstream types of immobilizer and initialization strategy, this paper researches an initial- ization adaptive strategy of immobilizer based on mixed-model production and applies it in the end of automobile production process, which can identify the type of immobilizer, lead the process of initialization, reduce the cost and improve the production efficiency.