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In order to research physical and chemical properties of blend diesel fuel,EL,biodiesel and n-butanol was taken as the key additive.Some experiments was studied based on China National Standards and Codes: GB/T25199-2010 biodiesel fuel blend(B5).The result showed that 1)There was no phase separation in all the alternative fuel formulas at 4℃; 2)The biodiesel which has a significantly higher kinematic viscosity,has the biggest effect to formulas,the second is EL; 3)The close-cup flash point of n-butanol-diesel blended fuel close to n-butanol,the content of diesel hardly cause any infection; 4)The 10%volume fraction distillation temperatureof n-butanol-diesel blends decrease significantly; 5)Adding the same ratio of EL had biggest influence of the density of blended fuel,6)The adding of biodiesel and high dose n-butanol cause slightly corrosiveness.