Rapamycin is an immunosuppressant drug that has been shown to inhibit cancer growth.The effect of Rapamycin on the metabalome of HEK 293 Human cancer cells is investigated in this study by interrogating the extracted metabolites by LC/QTOFMS.The extracted metabolites were separated on a SB-Aq column, which is a reverse phase column compatible with an aqueous mobile phase.This untargeted data is mined using a database of compounds found in a specific pathway.Such a database is created by extracting the accurate mass of compounds found in selected specific pathways.Such an approach helps to derive information from a metabolomics study about differential expression of compounds found in specific pathways.The differential expression analysis is performed by a chemometric software that also allows searching for biological pathways.