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[目的]调查临沂市健康人群中肠道病毒的隐性感染情况,追踪手足口病流行的传播因素,为制定手足口病预防和控制策略提供科学的依据。[方法]采用多阶段整群抽样方法,在临沂市选择2009年手足口病发病率高发县区和低发县区的城镇和农村托幼机构各一处,于4、6、9月各采集儿童及其监护人大便标本,用荧光定量PCR进行肠道病毒核酸检测。[结果]合计检测1 499人,阳性159人,阳性检出率为10.61%。阳性检出率,4月为7.50%、6月为15.06%、9月为9.31%(P<0.05);农村村民为9.20%,托幼儿童为12.56%;兰山区为13.12%,平邑县为7.35%(P<0.05);男性为12.67%,女性为10.26%。[结论]在健康人群中存在肠道病毒隐性感染情况,隐性感染率的高低与我市手足口病的地区分布、季节性变化是一致的。
[Objective] To investigate the latent infection of enterovirus in healthy population of Linyi and to trace the spread of HFMD epidemic, so as to provide a scientific basis for the prevention and control strategy of hand-foot-mouth disease. [Methods] A multi-stage cluster sampling method was used to select one place for urban nurseries and rural nurseries in 2009 with high prevalence of HFMD and low incidence in 2009 in Linyi City, collected in April, June and September Stool samples from children and their guardians were tested for enterovirus nucleic acid by fluorescent quantitative PCR. [Results] A total of 1 499 people were detected, 159 were positive, the positive rate was 10.61%. The positive detection rate was 7.5% in April, 15.06% in June and 9.31% in September (P <0.05). The rural residents were 9.20% and the children in kindergartens were 12.56%, while the Lanshan District was 13.12% Was 7.35% (P <0.05); 12.67% in males and 10.26% in females. [Conclusion] There is a latent infection of enterovirus in healthy population. The level of latent infection is consistent with the regional distribution and seasonal changes of HFMD in our city.