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目的:建立栀子及其炮制品中熊果酸的测定放法,考察栀子炮制前后熊果酸的含量变化。方法:采用高效液相色谱法,色谱柱为Agilent C18(4.6mm×150mm,5μm),流动相为流动相:甲醇-0.1%磷酸(86:14);检测波长220 nm。结果:熊果酸在0.120～1.200μg之间线性关系良好,平均加样回收率97.54%,RSD为1.8%。结论:方法用于栀子及其炮制品中熊果酸含量测定简单方便,重复性好。
OBJECTIVE: To establish a method for determination of ursolic acid in gardenia and its processed products, and to study the changes of ursolic acid content before and after processing gardenia. Methods: The mobile phase consisted of Agilent C18 (4.6 mm × 150 mm, 5 μm) and mobile phase consisted of methanol-0.1% phosphoric acid (86:14) and detection wavelength of 220 nm by high performance liquid chromatography. Results: Ursolic acid had a good linear relationship between 0.120 and 1.200 μg with an average recovery of 97.54% and a RSD of 1.8%. Conclusion: The method for determination of ursolic acid in Fructus Gardeniae and its processed products is simple and convenient, and has good reproducibility.