“京花1号”小麦的花药培养在含不同 NaCl浓度,附加 2mg/L 2,4-D和0.5mg/L KT的N_6培养基上,确定筛选浓度。0.3％、0.4％和0.5％ NaCl逆境下,接种经γ射线辐照的和未辐照的“京花1号”小麦花药,诱导愈伤组织,并在同样NaCl逆境下分化,获得6株花粉植株。其中经γ辐照,0.5％NaCl下获得的1株,得到1粒种子。经无盐条件下繁殖后,γH_3(照射第3代)种植在中等盐渍地,在0.29％含盐量土壤中生长正常。γH_4代按单株作进一步盐渍地、盐池和水培鉴定,有3个株行其耐盐力明显高于亲本“京花1号”。 Anther culture of “Jinghua 1” wheat was determined on N 6 medium containing different concentrations of NaCl, 2 mg / L 2,4-D and 0.5 mg / L KT. 0.3%, 0.4% and 0.5% NaCl were inoculated with γ -ray irradiated and non-irradiated “Jinghua 1” wheat anthers to induce callus and differentiate under the same NaCl stress conditions to obtain 6 pollen Plant. Among them, 1 strain was obtained by γ irradiation and 0.5% NaCl to obtain 1 seed. After propagating under salt-free conditions, γH_3 (the third generation of irradiation) was planted in medium salinity and grew normally in 0.29% salinity soil. γH_4 generation by individual salt for further salt ponds and hydroponic identification, 3 strains of its salt tolerance was significantly higher than the parent “Jinghua 1”.