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Objectives To examine in vivo interactions between angiotensin Ⅱ (Ang Ⅱ) AT1a receptor (AT1aR), angiotensin-converting enzymes (ACE) and ACE2 using small hairpin RNA (shRNA) gene-silencing methods in mice brainstem nucleus tractus solitarius (NTS). Methods C57BL mice (n=8) were used as animal model. Method of microinjection in the nucleus of NTS was adopted. After ten days, mice were killed and their brain tissue were fixed and sectioned. The expression levels of AT1aR, ACE and ACE2 mRNA at both sides of NTS were examined by in situ hybridization. Based on compared t-test, the changing for mRNA expression was examined. Results After the expression of AT1aR mRNA was significantly inhibited (61.6%±6.8%) by AT1aR-shRNA, it was associated with decreases in ACE2 mRNA expression from (1.05±0.12)μCi/mg to (0.74±0.09)μCi/mg (29.0%±14.5%, P<0.01) on the same side of the brainstem. ACE mRNA expression was consistent at both sides (0.50 μCi/mg±0.09 μCi/mg and 0.53 μCi/mg±0.08 μCi/mg), with insignificant difference (P>0.05). Conclusions The gene silencing result showed that there were interactions between brainstem AT1aR and ACE2.ACE mRNA expression was not altered by RNA interference treatment at AT1aR.
Objectives To examine in vivo interactions between angiotensin Ⅱ (Ang Ⅱ) AT1a receptor (AT1aR), angiotensin-converting enzymes (ACE) and ACE2 using small hairpin RNA (shRNA) gene-silencing methods in mice brainstem nucleus tractus solitarius Method of microinjection in the nucleus of NTS was adopted. After ten days, mice were killed and their brain tissues were fixed and sectioned. The expression levels of AT1aR, ACE and ACE2 mRNA Results from the expression of AT1aR mRNA was significantly inhibited (61.6% ± 6.8%) by AT1aR-shRNA, it was associated with decreasing in ACE2 mRNA expression from (1.05 ± 0.12) μCi / mg to (0.74 ± 0.09) μCi / mg (29.0 ± 14.5%, P <0.01) on the same side of the brainstem. consistent at both sides (0.50 μCi / mg ± 0.09 μCi / mg and 0.53 μCi / mg ± 0 .08 μCi / mg), with insignificant difference (P> 0.05). Conclusions The gene silencing result showed that there were were interactions between brainstem AT1aR and ACE2.ACE mRNA expression was not altered by RNA interference treatment at AT1aR.