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我们观察了正常人及慢性肾功能不全患者血尿β_2-微球蛋白(β_2-m)、α_1-m、Tamm-Horsfall蛋白(THP)、BUN、Cr水平变化,并探讨其临床意义。 材料和方法 一、对象: (一)正常对照组:35例(男17例,女18例),年龄21～71(46.25±14.12)岁,为健康体检者。 (二)慢性肾功能不全组:35例(男28例,女7例),年龄19～72)43.18±16.21)岁。均为本院住院病人。肾功能不全按1985年全国肾脏病学术会议诊断标准确诊。病程1月～20年(平均4.65±0.667)。原发病为:慢性肾小球肾炎17例,隐匿性肾炎9例,糖尿病肾病、肾结核各2例,间质性肾炎、痛风、高血压肾小动脉硬化、肾结石各1例。 二、方法:对受检者采取空腹静脉血,分离血清,置-20℃以下低温冰柜备测。 α_1-m试剂盒由天津九鼎医学生物工程公司提供。β_2-m、THP试剂盒由中国原子能研究所提供。肌酐用苦味酸法、尿素氮用酶法。 三、统计学处理;结果以±S表示,组间比较用t检验。 结果 一、慢性肾功能不全(CRF)患者血清和尿β_2-m、α_1-m较正常组明显升高(p<0.01),THP明显下降(p<0.01)见表1和表2。
We observed the changes of hematuria β_2-microglobulin, α_1-m, Tamm-Horsfall protein (THP), BUN, Cr levels in patients with chronic renal failure and normal controls and their clinical significance. Materials and methods First, the object: (a) normal control group: 35 cases (17 males and 18 females), aged 21-71 (46.25 ± 14.12) years old, for the healthy physical examination. (B) of chronic renal insufficiency group: 35 cases (28 males and 7 females), age 19 ~ 72) 43.18 ± 16.21) years old. All hospital patients. Renal insufficiency diagnosed by the diagnostic criteria of the National Kidney Disease Academic Conference in 1985. Duration of 1 month to 20 years (average 4.65 ± 0.667). Primary disease: 17 cases of chronic glomerulonephritis, occult nephritis in 9 cases, diabetic nephropathy, renal tuberculosis in 2 cases, interstitial nephritis, gout, hypertensive renal arteriosclerosis, kidney stones in 1 case. Second, the method: the subjects to take fasting venous blood, serum separation, set -20 ℃ below the freezer temperature measurement. α_1-m kit by Tianjin Jiuding Medical Biological Engineering Company. β_2-m, THP kit provided by China Institute of Atomic Energy. Creatinine picric acid method, urea nitrogen enzymatic method. Third, the statistical analysis; results to ± S said that the comparison between groups using t test. Results 1. Serum and urinary levels of β_2-m and α_1-m in serum and urine in patients with chronic renal failure (CRF) were significantly higher than those in the normal group (p <0.01), and THP was significantly decreased (p <0.01) in Table 1 and Table 2.