• 不 限
  • 期刊论文
  • 硕博论文
  • 会议论文
  • 报 纸
  • 英文论文
  2. 您的位置
  3. 首页
  4. 期刊论文
  5. Molecular Cloning and Expression of Human Interleukin-6 in Insect Cells

Molecular Cloning and Expression of Human Interleukin-6 in Insect Cells

来源 :中国科学：化学英文版 | 被引量 : 0次 | 上传用户：chentongxu
【摘 要】
：
670-bp hIL-6 cDNA fragments have been amplified by polymerase chain reaction(PCR)using recombinant plasmid pBMIL-6A as templates and two synthetic oligonucleoti
【作 者】
：
赵春文 王嘉玺 肖定华 马贤凯 
【机 构】
：
InstituteofBasicMedicalSciences
【出 处】
：
中国科学：化学英文版
【发表日期】
：
1994年9期
【关键词】
：
hIL-6 PCR BACULOVIRUS VECTOR wtAcMNPV Sf9 CELLS hIL-6PCRbaculovirus vectorwtAcMN 
【基金项目】
：
the National Natural Science Foundation of China
下载到本地 , 更方便阅读
下载此文 赞助VIP
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
670-bp hIL-6 cDNA fragments have been amplified by polymerase chain reaction(PCR)using recombinant plasmid pBMIL-6A as templates and two synthetic oligonucleotides containing the opti-mired translation initiation sequence/and restriction sites suitable fo
其他文献
The Role of Monosialoganglioside GM1 in LTP-Induction in Rat Hippocampal Slices
The effect of monosialoganglioside GM1 of different doses on the long-term potentiation (LTP) of synaptic transmission has been studied in the CA1 region of rat
期刊
MONOSIALOGANGLIOSIDEGM1HIPPOCAMPALSLICElong-termPOTENTIATION(LTP)rats.mo
Relationship between the induction of heat shock proteins and the decrease in glucocorticoid recepto
Previously,it has been found that glucocorticoid receptor(GR)binding activity decreasedrapidly during heat shock response in HOS-8603,a human osteosarcorna cell
期刊
heatshockproteinGLUCOCORTICOIDreceptoralkalinePHOSPHATASEQUERCETINOSTEOS
The crystal structure of deshexapeptide(B25-B30)insulin at 0.25 nm resolution
The determination of deshexapeptide(B25-B30)insulin(DHI)was divided into two steps.At the first step,the rough structure model of DHI molecule was determined by
期刊
deshexapeptideinsulin(DHI)molecularclose-packingmethod.deshexapeptide insul
Hormonal Regulation of Oocyte Development and Maturation of Amphioxus
Using the electron microscopic technique and radioimmunoassay,the hormonal regulationof the oocyte development and maturation of the amphioxus has been investig
期刊
AMPHIOXUSOOCYTEGONADOTROPINRELEASINGhormoneanalogueRADIOIMMUNOASSAYamphio
Interference of electromagnetic waves in dynamic metabolism
Life is a continuous process of the dynamic metabolism.The influence of electromagneticwaves on the process of metabolism cannot be neglected.Here a new theory
期刊
ELECTROMAGNETICINTERFERENCEDYNAMICMETABOLISMINTERFERENCEFACTORelectromagne
Rifting cycle and storeyed texture of copper deposits and their geochemical evolution in Kangdian re
Along with the evolution of the earth crust, in the south of Kangdian Axis the polycyclic rifting occurred: Ptd-Ptk, Jinshajiang continental margin sea type aul
期刊
KangdianAXISRIFTINGCYCLEstoreyedTEXTUREofcopperDEPOSITSevolutionofgeo
Synthesis, Crystal Structure, and Properties of [Cu（tren）（im）Zn（tren）]（ClO<sub>4</sub>）&
This paper reports the synthesis,properties and crystal structure of the title model compound with the ESR parameters g<sub>∥</sub> =2.007,g<sub>⊥</sub>=2.191
期刊
BESODmodelcompoundsCu(Ⅱ)-Zn(Ⅱ)BINUCLEARcomplexerystalstructure.BESOD mo
Magnetic property of Chinese loess and its paleoclimate significance
The magnetic minerals of paleosol unit S8 and loess unit L8 in Weman section have been studied. The experiments show that the remanent magnetization of Chinese
期刊
LOESS-PALEOSOLSEQUENCEPALEOCLIMATErockMAGNETISMWeinanShaanxi.loess-paleos
NaCl-induced conformational transition of the acid-unfolded state of staphylococcal nuclease
The acid-unfolded state(U_A)of staphylococcal nuclease(SNase)can fold into a state(Astate)in acidic solution of high NaCl concentration,that is,U_A→A.The trans
期刊
STAPHYLOCOCCALNUCLEASEMOLTENglobularSTATEacid-unfoldedSTATEstaphylococcal
Is the Chandler Period Stable?
The problem on the Chandler period is an unsolved one. Several authors suggested a hypothesis that the Chandler wobble is only one free period which slightly ch
期刊
ChandlerPERIODamplitude-dependentDECONVOLUTIONmethod.Chandler period ampli