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研制了以中性载体(ETH1097)为电活性物质的PVC钠离子选择性微电极。电极尖端直径小至2.5μm,线性响应范围10~(-3)-5×10~(-1)MNa~+,检测下限1.2×10~(-4)M,斜率为58.2mV(25℃).对K~+,Mg~(2+)、Ca~(2+)的选择性系数分别为0.050,6.5×10~(-4)及5.5×10~(-3)。该微电极特别适用于细胞内钠离子活度的测量。实验探讨和比较了癸二酸二丁酯(DBS)和邻苯二甲酸二正辛酯(DOP)为增塑剂的钠微电极的性能。研究了四苯硼钠(NaTPB)和硫氰酸钠(NaSCN)加入膜中后,电极性能的变化。为用于细胞内及临床微量试样中钠离子活度测量,电极采用DBS为增塑剂,并加入适量的NaTPB较宜。
A PVC sodium ion-selective microelectrode with neutral carrier (ETH1097) as the electroactive substance was developed. The tip diameter of the electrode was as small as 2.5μm, the linear response range was 10 -3 -5 × 10 -1 MNa ~ +, and the detection limit was 1.2 × 10 -4 M with a slope of 58.2 mV (25 ℃) The selectivity coefficients for K ~ +, Mg ~ (2 +) and Ca ~ (2+) were 0.050, 6.5 × 10 ~ (-4) and 5.5 × 10 ~ (-3), respectively. The microelectrode is particularly suitable for the measurement of intracellular sodium ion activity. The properties of sodium microelectrode with plasticizer were studied and compared with dibutyl sebacate (DBS) and di-n-octyl phthalate (DOP). The effects of sodium tetraphenylborate (NaTPB) and sodium thiocyanate (NaSCN) on the membrane properties were investigated. For the intracellular and clinical micro-sample sodium ion activity measurement, the electrode using DBS as a plasticizer, and appropriate amount of NaTPB is appropriate.