目的:探讨正常形态精子百分率对体外受精-胚胎移植(IVF-ET)治疗结局及新生儿的影响。方法:采用WHO严格标准法将精液标本分为3组:中度畸形组:正常形态精子百分率5%～10%,轻度畸形组:10%<正常形态精子百分率<15%,正常组:正常形态精子百分率≥15%,比较各组间正常受精率、卵裂率、优质胚胎率、种植率、临床妊娠率及新生儿情况。结果:各组间患者年龄(男、女方)差异不显著(P>0.05);中度畸形组正常受精率显著低于轻度畸形组(63.70%vs73.74%,P<0.05),但与正常组差异无统计学意义(63.70%vs68.05%,P>0.05);正常组的优质胚胎率最高,显著高于中度畸形组(44.83%vs35.75%,P<0.05),其他各指标3组间差异无统计学意义(P>0.05);280个移植周期共分娩125个婴儿,其中单胎分娩73例,双胎分娩26例,出生婴儿未见先天异常,3组间流产率、异位妊娠率、孕周、早产率、出生体重差异无统计学意义(P>0.05)。结论:正常形态精子百分率为5%～10%对常规体外受精的受精率无影响,但显著降低优质胚胎率,而10%<正常形态精子百分率<15%对常规体外受精治疗结局的各项指标均无明显影响;正常形态精子百分率在预测IVF-ET的助孕结局及新生儿情况方面存在一定局限性。 Objective: To investigate the effect of the percentage of normal spermatozoa on IVF-ET outcome and neonatal outcome. Methods: Semen samples were divided into three groups according to WHO strict standard method: moderate deformity group: 5% -10% normal sperm, mild deformity group: 10% normal sperm percentage: 15% normal group: normal Morphological sperm percentage≥15%. The normal fertilization rate, cleavage rate, high-quality embryo rate, implantation rate, clinical pregnancy rate and neonatal condition were compared between groups. Results: There was no significant difference in age (male and female) between the two groups (P> 0.05). Normal fertilization rate in moderate deformity group was significantly lower than that in mild deformity group (63.70% vs 73.74%, P <0.05) There was no significant difference between the normal group (63.70% vs68.05%, P> 0.05). The normal group had the highest rate of high quality embryo, which was significantly higher than the moderate deformity group (44.83% vs35.75%, P <0.05) There were no significant differences among the 3 groups (P> 0.05). A total of 125 babies were delivered in 280 transplant cycles, of which 73 were single births, 26 were twins, and no congenital abnormalities were found in infants. The abortion rate , Ectopic pregnancy rate, gestational age, premature birth rate, birth weight, no significant difference (P> 0.05). CONCLUSIONS: Normal sperm percentage of 5% ~ 10% has no effect on the fertilization rate of conventional in vitro fertilization but significantly reduces the rate of high quality embryo, while 10%