ＡＣＴＨ是垂体分泌的重要激素之一 ,测定血浆ＡＣＴＨ对下丘脑 垂体 肾上腺轴功能的评价具有重要意义。制备ＡＣＴＨ单克隆抗体 ,以期建立ＡＣＴＨ ＩＲＭＡ方法。ＡＣＴＨ或其片段与ＢＳＡ经碳化二亚胺偶联剂联接后制备免疫原 ,初次免疫为完全福氏佐剂乳化后在背部皮下多点 ACTH is one of the important hormones secreted by the pituitary. It is of great significance to evaluate the role of plasma ACTH on the hypothalamic pituitary adrenal axis. ACTH monoclonal antibody was prepared in order to establish the ACTH IRMA method. ACTH or its fragments and BSA carbodiimide coupling agent prepared after the immunogen, the initial immunization complete Freund’s adjuvant emulsified in the back of the subcutaneous multi-point