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对在6个月内从121名产科新生儿中的30名检出的31株铜绿假单胞菌进行RAPD指纹图谱分析和血清学分型。24株为O:6血清型,6株属于另外3个血清型,1株未能分型。RAPD分型率为100%。31个菌株分成5个RAPD谱型。25株为R:1型,其它6株为另外的4个谱型。血清型不同的新生儿菌株的RAPD谱型亦不相同。2株O:1型的菌株,其RAPD谱型却不同。同期分别来自4个其他科室的5名患者的5株O:6型PA菌的RAPD谱型各自不同,与新生儿O:6型的RAPD谱型也不同。结果表明,铜绿假单胞菌在产科新生儿的暴发流行,O:6/R:1型为暴发流行菌株;RAPD分型率高,分辨力强,快速简便,颇具医院感染分子流行病学研究的应用价值。
RAPD fingerprinting and serological typing of 31 strains of Pseudomonas aeruginosa detected from 30 out of 121 obstetric neonates within 6 months were performed. 24 strains were O: 6 serotypes, 6 strains belonged to the other 3 serotypes, and 1 strain failed to type. RAPD typing rate was 100%. 31 strains were divided into 5 RAPD patterns. 25 strains were R: 1 type, the other 6 strains were the other 4 types. The RAPD patterns of different serotype neonates are also different. Two O: 1 strains showed different RAPD patterns. Five strains of O: 6 PA from five patients in four other departments in the same period had different RAPD patterns, which was different from that of neonatal O: 6 RAPD patterns. The results showed that Pseudomonas aeruginosa outbreak in neonatal obstetrics, O: 6 / R: 1 outbreak strains; RAPD high typing rate, resolution, quick and easy, quite a molecular epidemiology of nosocomial infection The application value.