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目的:探索μ阿片受体(MOR)在大鼠结肠肠肌丛的分布及化学神经解剖学特性。方法:取大鼠结肠右曲和左曲之间肠管行全层铺片,剥离粘膜层、粘膜下层和环形肌层,保留纵行肌层。运用免疫荧光组织化学双重标记技术染色,共聚焦显微镜下观察MOR在大鼠结肠肠肌丛的分布及与一氧化氮合酶(NOS)、肠血管活血肽(VIP)、P物质(SP)或降钙素基因相关蛋白(CGRP)等在肠神经细胞的共存关系。结果:免疫荧光组织化学染色显示:在大鼠结肠肠肌层,MOR阳性细胞聚集形成神经节,其阳性突起穿行于神经节之间形成网格状神经丛。在肠肌丛神经细胞内,可见MOR分别与NOS、VIP、SP、CGRP共存。MOR阳性神经纤维和终末分布于NOS阳性神经细胞周围,也可见部分NOS、VIP阳性神经纤维和终末分布于MOR阳性神经细胞表面。有大量SP和CGRP阳性神经纤维穿行于MOR阳性神经细胞之间并包绕于MOR阳性神经细胞的胞体周围。结论:在结肠的肠肌丛,MOR与抑制性和感觉性神经递质在肠神经细胞有共存,且相互之间有纤维联系,推测MOR可能介导了阿片类调节肠神经细胞内抑制性神经递质的释放,并可能对肠感觉信息的传递有调节作用。
Objective: To explore the distribution and chemical neuroanatomy of mu opioid receptor (MOR) in the colonic myenteric plexus of rats. Methods: The intestine of right colon and left intestine of rats was implanted with full-layer intestine to peel off the mucosa, submucosa and ring muscle, keeping the longitudinal muscle layer. Immunofluorescence staining was used to observe the distribution of MOR in colonic myenteric plexus of colon and the expression of MOR and the expression of nitric oxide synthase (NOS), vasoactive intestinal peptide (VIP), substance P (SP) or Calcitonin gene-related protein (CGRP) in the coexistence of enteric neurons. Results: Immunofluorescence histochemical staining showed that in the colonic muscle of the colon, MOR-positive cells gathered to form ganglia, and the positive neurites protruded from the ganglia to form a grid-like plexus. In the myenteric plexus nerve cells, we can see MOR and NOS, VIP, SP, CGRP coexistence. MOR positive nerve fibers and terminal distribution in the NOS positive nerve cells around the part of the visible NOS, VIP positive nerve fibers and terminals distributed in the MOR positive nerve cell surface. A large number of SP and CGRP positive nerve fibers traverse between MOR positive nerve cells and surround the soma of MOR positive neurons. CONCLUSIONS: MOR and inhibitory and sensory neurotransmitters coexist in enteric neurons in the colon myenteric plexus, and there is a fiber connection between them. It is speculated that MOR may mediate opioid regulation of inhibitory nerves in enteric neurons Delivery of neurotransmitters, and may regulate the delivery of intestinal sensory information.