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目的:监测兔急性肺血栓栓塞症(APTE)模型血清肿瘤坏死因子-α(TNF-α),(白细胞介素-4,1β)IL-4,IL-1β水平的变化,探讨炎症因子与肺损伤的关系。方法:自体血栓法建立兔APTE模型,随机分为对照组、模型组。ELISA法检测血清各指标,术毕行病理分析。结果:栓塞后IL-4在发病早期未见升高,发病后6 h明显升高,模型组与对照组分别为(42.8±1.35),(16.9±1.59)ng·mL-1(P<0.05),栓塞后4 hTNF-α模型组与对照组分别为(3.52±0.42),(1.29±0.36)ng·mL-1(P<0.05),栓塞后2 h IL-1β模型组与对照组分别为(2.66±0.12),(0.34±0.15)ng·mL-1(P<0.05),水平明显高于对照组。形态学观察结果可见,栓塞后肺动脉内血栓形成,肺组织萎缩、出血、炎性反应明显。结论:APTE模型血清炎症因子发生的不同变化,在引起急性肺损伤中起重要作用。
Objective: To observe the changes of serum levels of tumor necrosis factor-α (TNF-α), interleukin-4 (IL-4) and IL-1β in rabbits with acute pulmonary embolism (APTE) Injury relationship. Methods: Rabbit APTE model was established by autologous thrombosis, and randomly divided into control group and model group. ELISA method for the detection of serum indicators, surgery line pathological analysis. Results: After embolization, IL-4 did not increase in the early stage of the disease, and increased significantly at 6 h after onset of the disease. The levels of IL-4 in the model group and the control group were (42.8 ± 1.35) and (16.9 ± 1.59) ng · mL- (3.52 ± 0.42) and (1.29 ± 0.36) ng · mL-1, respectively (P <0.05) at 4 h after embolization. At 2 h after embolization, IL-1β model group and control group (2.66 ± 0.12) and (0.34 ± 0.15) ng · mL-1, respectively (P <0.05), which was significantly higher than that of the control group. Morphological observation shows that pulmonary embolism after thrombosis, lung tissue atrophy, bleeding, inflammatory response. Conclusion: The different changes of serum inflammatory factors in APTE model play an important role in causing acute lung injury.