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利用细胞学方法、种子储藏蛋白电泳技术和抗病接种鉴定对四川小麦地方品种与非洲黑麦 (S .africanum)人工合成双二倍体进行了研究 ,结果表明 :①根尖细胞的染色体计数发现 ,来自非洲黑麦的随体在双二倍体中仅得到部分表达 ;Giemsa C带分析能准确鉴定双二倍体中的非洲黑麦染色体 ,发现非洲黑麦与栽培黑麦的染色体C带有较大的区别 ;②种子醇溶蛋白电泳发现除非洲黑麦的聚集黑麦碱蛋白在双二倍体中不表达外 ,其余来自普通小麦和非洲黑麦的相应蛋白带能够在双二倍体中正常表达 ;③双二倍体及其亲本的苗期抗白粉病性和成株期抗条锈性分析表明 ,非洲黑麦对这两种病害的优良抗性在双二倍体中并不表达 ,抗性受到普通小麦背景的抑制。另外本文对小麦 -外源染色体组的相互作用和该双二倍体在小麦与八倍体小黑麦育种中的应用进行了讨论
Cytological methods, seed storage protein electrophoresis technology and disease-resistant inoculation were used to study the synthetic diploid of Sichuan wheat cultivars and African rye (S.africanum). The results showed that: (1) The chromosome counts of root tip cells were found , The satellites from African rye were only partially expressed in the diploid diploid; the Giemsa C-band analysis was able to accurately identify the African rye chromosomes in the diploid and found that chromosome C in African rye and cultivated rye The difference was significant; ②Water prolamin electrophoresis found that in addition to the rye aggregates of rye base protein is not expressed in the diploid, the other from the common wheat and African rye corresponding protein bands in the diploid ; ③ The diploid and its parents at seedling stage showed resistance to powdery mildew and adult stripe rust resistance analysis showed that the excellent resistance of African rye to the two diseases did not occur in diploid Expression and resistance were inhibited by the common wheat background. In addition, this paper discusses the interaction of wheat-exogenous genome and the application of the diploid in the breeding of wheat and octoploid triticale