本文制定了用α，β，γ，δ－四苯基卟啉分光光度法测定血液和头发中痕量铜。本法操作简便，灵敏而有选择性，摩尔吸光系数为4.5 × 105。用十二烷基磺酸钠作胶束增溶剂，加入四甲基溴化铵作胶束稳定剂，采用焦磷酸钠掩蔽血液中大量的铁并提高了锌的允许量。实验了血液和头发中十二种主要元素的干扰情况。应用本法对几种血液和头发样品进行测定，均得到满意的结果。 This paper developed a, β, γ, δ-tetraphenyl porphyrin spectrophotometric determination of trace amounts of copper in the blood and hair. This method is simple, sensitive and selective, with a molar extinction coefficient of 4.5 × 105. With sodium dodecyl sulfate as micellar solubilizer, tetramethylammonium bromide was added as a micellar stabilizer, and sodium pyrophosphate was used to mask large amounts of iron in the blood and to increase the allowable amount of zinc. Experiment with the interference of twelve major elements in blood and hair. The application of this method on several blood and hair samples were measured, are satisfactory results.